Associate Professor

Duke University

Department of Mechanical Engineering

and Materials Science

Hudson Hall 188

Durham, NC 27708

Telephone:  (919) 660 5381

E-mail:  pemar@duke.edu

Research Interests

Nanomechanics of single molecules and mesoscopic-scale systems.  Force-induced conformational transitions in single molecules by Atomic Force Microscopy and computational approaches (ab initio/MD). Nano(bio)technology.

Education

1985                                MS in Physics (biophysical specialization), Faculty of Physics, University of Warsaw, Poland

1991                Ph.D. in Electrical Engineering, Electrotechnical Institute/Polish Academy of Sciences, Warsaw, Poland

Work Experience

Aug.2002-present      Associate Professor, Department of Mechanical Engineering and Materials Science, Duke University

1998-2002                   Assistant Professor of Biophysics, Mayo Medical and Mayo Graduate School, Biomedical Engineering Track, Mayo Clinic and Foundation

1996-present               Associate Consultant, Department of Physiology and Biophysics, Mayo Clinic and Foundation, Rochester, MN

1991-1996                   Adiunkt, Electrotechnical Institute/Polish Academy of Sciences, Warsaw, Poland (January 1992 - June 1996 on leave)

1993-1996                   Research Fellow, Department of Physiology and Biophysics, Mayo Clinic, Rochester, MN

1992-1993                       Postdoctoral Associate, Department of Biochemistry, University of

Minnesota, St. Paul, MN

1991-1992                       Visiting Scientist, Saitama University, Urawa City, Japan

1985-1991                   Junior, then senior, research assistant in the Department of Fundamental Research in Electrotechnics of the Polish Academy of Sciences and of the Electrotechnical Institute, Warsaw, Poland

1989-1990                       Fulbright Fellow, Department of Biochemistry, University of Minnesota

Teaching

Jan.-May 2003   Course: ME101 Thermodynamics, Duke University

January 2001              Course:  Quantitative Biology I (Molecular biophysics), Mayo Clinic (course director)

February 2000             Course:  Quantitative Biology I, Mayo Clinic (course co-organizer)

Jan.-March 1999         Course:  Quantitative Biology I, 12-week course, Physiology and Biophysics Department, Mayo Clinic (course co-organizer)

April-June 1998           Course:  Bphy 8350, “Concepts in Molecular Biophysics,” 12-week course, Physiology and Biophysics Department, Mayo Clinic (course co‑organizer)

Grant Awards

2001                National Science Foundation Grant Award MCB-0110093, “Force-induced Conformational Transitions in Single Polysaccharide Molecules by AFM,”
(Principal Investigator) 60 months.

2000                                National Institute of Health Grant Award HL 66030, “Micromechanics of the Extracellular Matrix,” (PI: J.M. Fernandez), 60 months.

1999                                National Institute of Health Grant Award HL 61228, “The Molecular Basis of Titin Elasticity,” (PI: J.M. Fernandez), 48 months.

1998                                National Science Foundation Grant Award MCB-9808310, “Force-induced Conformational Transitions in Single Polysaccharide Molecules by AFM,” (Principal Investigator) 36 months.

Awards and Honors

1991                Distinguished Doctoral Dissertation, Electrotechnical Institute, Warsaw, Poland

1991                Japan International Cooperation Agency Scholarship, Saitama University,
Urawa City, Japan

1989                Fulbright Scholarship, University of Minnesota, St. Paul, MN, USA

1987-1989       Doctoral Fellowship, Electrotechnical Research Institute, Warsaw, Poland

1987                Grant to attend and present a paper at the 9th International Symposium on Bioelectrochemistry and Bioenergetics, Szeged, Hungary

Service

Nanotechnology Science and Engineering; Biosystems at the Nanoscale, National Science Foundation. March  11-12, 2002, Arlington, VA.

Nanotechnology Science and Engineering; Biosystems at the Nanoscale, National Science Foundation. February 24-26, 2003, Arlington, VA.

Membership in Professional and Scientific Societies:

The Biophysical Society (USA)

American Chemical Society

The Applied Electromagnetism Society (Poland)

Invited Seminars to Univeristies and Research Institutes

University of Illinois, Beckman Institute

Keio University, Department of Physics, Faculty of Science and Technology

Kyoto University, Institute for Chemical Research

Saitama University, Department of Electrical Engineering

Nencki Institute of Experimental Biology

Lublin Polytechnics

Nicolas Copernicus University

Wyeth Biotechnology Discovery Research Group

Dartmouth College

University of Wisconsin

Arizona State University

University of Minnesota

Duke University

National Center for Agricultural Utilization Research (USA)

Meetings

June 15-16, 2003               NSF workshop on Nanoscale Mechanical Engineering, Arlington, VA

Aug. 18-22, 2002          American Chemical Society, Boston, MA

April 7-11, 2002            American Chemical Society, Orlando, FL

February 23-27, 2002   Biophysical Society, San Francisco, CA

April 1-5, 2001                American Chemical Society, San Diego, CA

Feb. 17-21, 2001            Biophysical Society, Boston, MA

October 19-21, 2000      Biophysics Triangle Meeting, Durham, North Carolina

April 16-17, 2000            “Parallel MD Development and Use -- Challenges and Opportunities,” University of Illinois, Champaign-Urbana, Illinois

Feb. 12-16, 2000            Biophysical Society, New Orleans, Louisiana

Oct. 13-16, 1999            The BMES-EMBS (Biomedical Engineering Society, Engineering in Medicine and Biology Society) 1st Joint Conference, Atlanta, GA

Oct. 1-2, 1999                First Annual Mayo Research Forum: From Molecules to Mankind, Rochester, MN

March 13-16, 1999         The 4th Johns Hopkins Protein Folding Meeting, Coolfont, WV

Feb. 13-17, 1999            Biophysical Society, Baltimore, MD

Dec. 12-16, 1998           The American Society for Cell Biology, San Francisco, CA

Feb. 22-26, 1998            Biophysical Society Annual Meeting, Kansas City, MO

August 8-10, 1996          International Symposium on Mechanisms of Biological Membrane Fusion, Noordwijkerhout, The Netherlands

Feb. 17-21, 1996            Biophysical Society, “Kinetics of the release of serotonin from Isolated secretory granules,” Baltimore, MD

March 6-10, 1994           38th Annual Meeting, Biophysical Society, New Orleans, LA

February 1990                34th Annual Meeting, Biophysical Society, Baltimore, MD

October 1988                4th International Frumkin Symposium “Bioelectrochemistry Today and Tomorrow,” Moscow-Suzdal, Russia

Sept. - Oct. 1988           The 5th International Symposium on Biophysics of Cell Surface, Kuhlungsborn, Germany

May 1988                        IX International School on Biophysics of Membrane Transport, Polanica-Zdroj, Poland

September 1987            9th International Symposium on Bioelectrochemistry and Bioenergetics, Szeged, Hungary

Bibliography

 (The total of 45 papers)

Marszalek, P.E., Oberhauser, A.F., Li, H. & Fernandez, J.M. (2003). The force driven conformations of heparin studied with single molecule force microscopy. Biophysical Journal, in press.

Carrión-Vázquez, M., Li, H., Lu, H., Marszalek, P.E., Oberhauser, A.F. & Fernández, J.M. (2003).  The mechanical stability of ubiquitin is linkage dependent. Nature Structural Biology, in press.

Li, H., Linke, W.A., Oberhauser, A.F.  Carrion-Vazquez, M., Kerkvliet, J.G., Lu, H.,  Marszalek, P.E. & Fernandez, J.M. (2002).  Reverse engineering of the giant muscle protein titin.  Nature 418, 998-1002.

Marszalek, P.E., Li, H., Oberhauser, A.F. & Fernandez, J.M. (2002).  Chair-boat  transitions in single polysaccharide molecules observed with force-ramp AFM.  Proceedings of the National Academy of Sciences (USA).  99, 4278-4283.

Marszałek, P.E. (2002) Chemiczna identyfikacja pojedynczych makromolekuł przy pomocy mikroskopy sił atomowych (AFM). Postępy Fizyki (in Polish, in press).

Marszalek, P.E., Li, H. & Fernandez, J.M. (2001).  Fingerprinting polysaccharides with single molecule AFM.  Nature Biotechnology  19, 258-262.

Marszalek, P. & Gimsa, J. (2000) Electroporation in AC and Rotating Electric Field. In Principles and applications of cell electroporation and electrofusion. Eds. Hemu Wang & Tingdong Xie, Tianjin Science and Technology Publishing Office. pp. 147-161.

Li, H., Carrion-Vazquez, M., Oberhauser, A.F., Marszalek, P.E. & Fernandez, J.M. (2000).  Point mutations alter the mechanical stability of immunoglobulin modules.  Nature Structural Biology  7, 1117-1120.

Fisher, T.E., Marszalek, P.E. & Fernandez J.M. (2000).  Stretching single molecules into novel conformations using the atomic force microscope.  Nature Structural Biology  7, 719-724.

Fisher, T. E., Carrion-Vazquez, M., Oberhauser, A.F., Li, H., Marszalek, P.E. & Fernandez, J.M. (2000).  Single molecule force spectroscopy of modular proteins in the nervous system.  Neuron  27, 435-446.

Carrión-Vázquez, M., Oberhauser, A.F., Fisher, T.E., Marszalek, P.E., Li, H. & Fernández, J.M. (2000).  Mechanical design of proteins studied by single-molecule force spectroscopy and protein engineering.  Prog. in Biophys. Mol. Biol.  74, 63-91.

Marszalek, P.E., Greenleaf, W.J., Li, H., Oberhauser, A.F., & Fernandez, J.M. (2000).  AFM captures quantized plastic deformations in gold nanowires.  Proceedings of the National Academy of Sciences of the USA  97, 6282-6286.

Marszalek, P.E., Lu, H., Li, H., Carrion-Vazquez, M., Oberhauser, A.F., Schulten, K., & Fernandez, J.M. (1999).  Mechanical unfolding intermediates in titin modules.  Nature  402, 100-103.

Marszalek, P.E., Pang, Y-P., Li, H., El Yazal, J., Oberhauser, A.F, & Fernandez, J.M. (1999).  Atomic levers control pyranose ring conformations.  Proceedings of the National Academy of Sciences of the USA  96, 7894-7898.

Marszalek, P.E., Oberhauser, A.F., Pang, Y.-P., & Fernandez, J.M. (1998).  Polysaccharide elasticity governed by chair-boat transitions of the glucopyranose ring.  Nature  396, 661-664.

Carrion-Vazquez, M., Marszalek, P.E., Oberhauser, A.F., & Fernandez, J.M. (1999).  AFM Captures length phenotypes in single proteins.  Proceedings of the National Academy of Sciences of the USA  96, 11288-11292.

Oberhauser, A.F., Marszalek, P.E., Carrion-Vazquez, M., & Fernandez, J.M. (1999). 

Single proteins misfolding events captured by AFM.  Nature Structural Biology  6, 1025‑1028.

Fisher, T.E., Marszalek, P.E., Oberhauser, A.F., Carrion-Vazquez, M., & Fernandez, J.M. (1999).  The micro-mechanics of single molecules studied with atomic force microscopy.  J. Physiology  520, 5-14.

Fisher, T.E., Oberhauser, A.F., Carrion-Vazquez, M., Marszalek, P.E., & Fernandez, J.M. (1999).  The study of protein mechanics with the atomic force microscope.  TIBS  24, 379-384.

Carrion-Vazquez, M., Oberhauser, A.F., Fowler, S.B., Marszalek, P.E., Broedel, S.E., Clarke, J., & Fernandez, J.M. (1999).  Mechanical and chemical unfolding of a single protein:  A comparison.  PNAS  96, 3694-3699.

Oberhauser, A.F., Marszalek, P.E., Erickson, H.P., & Fernandez, J.M. (1998).  The molecular elasticity of tenascin, an extracellular matrix protein.  Nature  393, 181-185. 

Marszalek, P.E., Farrell, B., Verdugo, P., & Fernandez, J.M. (1997).  Kinetics of release of serotonin from isolated secretory granules.  (I)  Amperometric detection of serotonin from electroporated granules.  Biophys. J.  73, 1160-1168.

Marszalek, P.E., Farrell, B., Verdugo, P., & Fernandez, J.M. (1997).  Kinetics of release of serotonin from isolated secretory granules.  (II)  Ion exchange determines the diffusivity of serotonin.  Biophys. J.  73, 1169-1183.

Marszalek, P.E. (1997).  Zasada dzialania diody zbudowanej z 'inteligentnego' polimeru.  Przeglad Elektrotechniczny, (in Polish).

Marszalek, P.E. (1997).  Mechanizm rektyfikacji pradw jonowych przez mikroczastki zelu.  Prace Instytutu Elektrotechniki, (in Polish).

Marszalek, P.E. (1996).  Granica faz zel/elektrolit - elektrochemiczny odpowiednik zlacza N-P.  Folia Societatis Scientiarum Lublinensis, 5, 30-46, (in Polish).

Xie, T.D., Chen, Yi-der, Marszalek, P., & Tsong, T.Y. (1997).  Fluctuation-driven directional flow in biochemical cycle:  further study of electric activation of Na,K pumps.  Biophys. J.  72, 2496-2502.

Marszalek, P.E., Farrell, B., & Fernandez, J.M. (1996).  Ion exchange gel regulates neurotransmitter release through the exocytotic fusion pore.  J. Gen. Physiol.  Society of General Physiologists Series, 51, 211-222.

Marszalek, P.E., Markin, V.S., Tanaka, T., Kawaguchi, H., & Fernandez, J.M. (1995).  The secretory granule matrix-electrolyte interface:  a homologue of the p-n rectifying junction.  Biophys. J.  69, 1218-1229.

Marszalek, P.E., Markin, V.S., Tanaka, T., Kawaguchi, H., & Fernandez, J.M. (1995).  Pulsed laser imaging demonstrates the mechanism of current rectification at a hydrogel interface.  Langmuir  11, 4196-4198.

Marszalek, P., & T.Y. Tsong. (1995).  Cell fission and formation of mini cell bodies by high frequency alternating electric field.  Biophys. J.  68, 1218-1221.

Xie, T.D., Marszalek, P., Chen, Yi-der, & Tsong, T.Y. (1994).  Recognition and processing of randomly fluctuating electric signals by Na, K-ATPase.  Biophys. J.  67, 1247-1251.

Pawlowski, P., Szutowicz, I., Marszalek, P., & Fikus, M. (1993).  Bioelectrorheological model of the cell.  5.  Electrodestruction of cellular membrane in alternating electric field.  Biophys. J.  65, 541-549.

Gimsa, J., Lowe U., Marszalek, P., & Tsong, T.Y. (1993).  Electroporation of cell membranes by rotating electric fields.  In:  Electricity and Magnetism in Biology and Medicine, ed., M. Blank, San Francisco Press, pp. 144-146.

Marszalek, P., Ido, S., & Krawczyk, A. (1992).  Review of the development of negative ion sources.  Publications of Electrotechnical Res. Institute  172, 34-60.

Gimsa, J., P. Marszalek, V. Loewe, & T.Y. Tsong. (1992).  Electroporation in rotating electrical fields.  Bioelectrochemistry and Bioenergetics  29, 81-89.

Gimsa, J., P. Marszalek, U. Loewe, & T.Y. Tsong. (1991).  Dielectrophoresis and electrorotation of neurospora slime and murine myeloma cells.  Biophys. J.  60, 749‑760.

Marszalek, P., Zielinski, J.J., Fikus, M., & Tsong, T.Y. (1991).  Determination of electric parameters of cell membranes by a dielectrophoresis methods.  Biophys. J.  59, 982-987.

Marszalek, P., Liu, D.-S., and Tsong, T.Y. (1990).  Schwan equation and transmembrane potential induced by alternating electric field.  Biophys. J.  58, 1053-1058.